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TCSPC時域法時間分辨熒光光譜儀ChronosBH

ChronosBH是一款時域法 (TCSCP) 緊湊型熒光光譜儀，用于在皮秒至秒這樣較寬的時間尺度范圍進行熒光衰變時間測量。該儀器的設計采用T式樣幾何結(jié)構(gòu)，可用于在兩個熒光發(fā)射通道上同時采集信號。各種脈沖光源都可以與ChronosBH連接，其中包括半導體激光器、LED,超連續(xù)激光器和超快激光器。探測器可從快速PMT、混合型PMT和砷化鎵 (GaAs) PMT，SPAD中選擇。該儀器還可以被升級，升級后能使用氙燈進行穩(wěn)態(tài)熒光測量。

▏產(chǎn)品詳情

簡介︰

ChronosBH的操作是全自動的；儀器控制、數(shù)據(jù)采集及處理是通過多維光譜學軟件Vinci完成的。

ChronosBH的關鍵特征

?擁有靈活的儀器結(jié)構(gòu)和兼容各種激發(fā)光源；

?占地面積小，為了在兩個通道上同時測量而設計了T式樣幾何形狀；

?短至皮秒上至數(shù)秒的壽命測量范圍且用同一激發(fā)光源和數(shù)據(jù)采集技術；

?全自動化的儀器部件包括了：比色皿支架、偏振片、濾光輪、單色器和攪拌器等；

?由計算機控制可將恒溫浴、滴定儀、停流儀器和高壓泵集成；

?可升級使其包括穩(wěn)態(tài)測試功能如激發(fā)光譜/發(fā)射光譜等

?用于快速且精確偏振測定的平行光束光路設計；

主要參數(shù)規(guī)格：

激發(fā)光源可選	♦皮秒脈沖半導體激光器： (nm): 370, 405, 436, 473, 635, 690, 780, 830 ♦納秒LED (nm): 280, 300, 335, 345, 460, 500, 520 ♦其他脈沖激光器：超連續(xù)譜光源、皮秒飛秒固體激光器等
激發(fā)和收集光路幾何結(jié)構(gòu)	平行光束設計，用于精準偏振測量
偏振片	UV級別格蘭-湯普遜偏振器，L/A = 2.0
探測器	♦PMT ♦混合型PMT, SAPD等
波長范圍	185 - 1,700nm (取決于探測器)
數(shù)字TCSPC模塊	♦最小時間窗口寬度 (ps): 1 ♦時間分辨率，RMS抖動 (ps): 34 ♦計數(shù)率：8.5 MHz
壽命測量范圍	100皮秒至數(shù)秒用同一激發(fā)光源和數(shù)字TCSPC信號處理單元；
軟件適用操作系統(tǒng)	Windows 10
電源要求	110 - 240 V, 50/60 Hz, 400 VAC
尺寸重量	540 mm (長) x 425 mm (寬) x 235 mm (高) 25 (kg)

ChronosBH時間分辨熒光光譜儀同PC1熒光光譜儀一樣，同下列ISS生產(chǎn)提供的樣品倉完美，參見資料下載中的文件。

　　 典型應用

　　材料的光致發(fā)光

　　免疫分析側(cè)試

　　納米粒子和上轉(zhuǎn)換粒子測試

　　蛋白質(zhì)折疊

　　DNA-蛋白質(zhì)的相互作用

　　小分子合成催化

測量實例

丙二醇中的熒光素

　　丙二醇中的熒光素的頻域各向異性衰減 (不同偏振相角和振幅比) ，在 470-nm (氙燈)下測試，發(fā)射線收集使用的是 OG530 高通濾波器。計算值r &thera; = 5.3 ns ， R0 = 0.40 和 τ = 4 ns, T = 27-28°C.

BodipyFL（氟硼二吡咯）水溶液

　　BodipyFL（氟硼二吡咯）水溶液的頻率響應 (相位和調(diào)制) ，在 471-nm激光二極管。發(fā)射線收集使用的是 520KV高通濾波器。數(shù)據(jù)的最佳擬合呈現(xiàn)指數(shù)衰減方式，衰減時間5.87 ns (x2 = 0.97).

時域強度衰減Time-Domain Intensity Decay

　　在 405-nm 脈沖激光二極管測試紙張的時域強度衰減。數(shù)據(jù)的最佳擬合呈現(xiàn)指數(shù)衰減方式，衰減時間 0.94 ns (x2 = 1.25).

　　溶于乙二醇

時域各向異性衰減Time-Domain Anisotropy Decay

　　在447nm脈沖激光二極管下溶于乙二醇溶液的Coumarin 6 （香豆素）的時域各向異性衰減。發(fā)射線通過 KV 505高通濾波器收集。計算值 θ = 2.6 ns ， R0 = 0.38 和 τ = 2.3 ns, T = 20-21°C.

應用領域及發(fā)表應用期刊

生物化學&分子生物學(細胞膜，核酸，蛋白質(zhì))

	Hydroxymethylation of DNA Influences Nucleosomal Conformation and Stability in Vitro. Mendonca, A., Chang, E.H., Liu, W., Yuan, C. Biochim Biophys Acta., 2014, 1839(11), 1323-9.
	Solution Scattering and FRET Studies on Nucleosomes Reveal DNA Unwrapping Effects of H3 and H4 Tail Removal. Andresen, K., Jimenez-Useche, I., Howell, S.C., Yuan, C., Qiu, X. PLoS One., 2013, 8(11), e78587.
	DNA Methylation Regulated Nucleosome Dynamics. Jimenez-Useche, I., Ke, J., Tian, Y., Shim, D., Howell, S.C., Qiu, X., Yuan, C. Sci Rep., 2013, 3, 2121.
	Clipping of Flexible Tails of Histones H3 and H4 Affects the Structure and Dynamics of the Nucleosome Nurse, N.P., Jimenez-Useche, I., Smith, I.T., Yuan, C. Biophys J., 2013, 104(5), 1081-8.
	The Effect of DNA CpG Methylation on the Dynamic Conformation of a Nucleosome Jimenez-Useche, I., Yuan, C. Biophysical Journal, 2012, 103, 2502-2512.
	Conformational Dynamics of Titin PEVK Explored with FRET Spectroscopy Huber, T., Grama, L., Hetényi, C., Schay, G., Fül?p, L., Penke, B., Kellermayer, M.S.Z. Biophysical Journal, 2012, 103(7), 1480-1489.

光合作用

Lessons From Chlorophylls: Modifications of Porphyrinoids Towards Optimized Solar Energy Conversion.
Karcz, D., Boron, B., Matwijczuk, A., Furso, J., Staron, J., Ratuszna, A., Fiedor, L.
Molecules., 2014, 19(10), 15938-54.

物理化學

	Photophysical Characterization of [Ir(ppy)2(dmb)][PF6] Towards Application in Light-Emitting Electrochemical Cells (LECs) Zanoni, K.P., Sanematsu, M., Murakami Iha, N.Y. Inorg Chem, 2014, 43, 162-164.
	Solid State Molecular Device Based on a Rhenium(I) Polypyridyl Complex Immobilized on TiO2 Films Patrocinio, A.O., Frin, K.P., Murakami Iha, N.Y. Inorg. Chem., 2013, 52, 5889-5896.
	A Pyrene Maleimide with a Flexible Linker for Sampling of Longer Inter-Thiol Distances by Excimer Formation Niwayama, S., Kassar, A.S., Zhao, T., Sutton, R.B., Altenberg, G.A. PLoS ONE, 2011, 6(10).

診斷學

	A Dual-Modality Optical Biopsy Approach for In Vivo Detection of Prostate Cancer in Rat Model Sharma, V., Patel, N., Shen, J., Tang, L., Alexandrakis, G., Liu, H. Journal of Innovative Optical Health Sciences, 2011, 4(3), 269-277.
	Auto-fluorescence Lifetime and Light Reflectance Spectroscopy for Breast Cancer Diagnosis: Potential Tools for Intra-Operative Margin Detection Sharma, V., Shivalingaiah, S., Peng., Y., Euhus, D., Gryczynski, Z., Liu, H. Biomed Opt Express, 2012, 3(8), 1825-1840.

化學&分子生物學(細胞膜，核酸，蛋白質(zhì))

	Hydroxymethylation of DNA Influences Nucleosomal Conformation and Stability in Vitro. Mendonca, A., Chang, E.H., Liu, W., Yuan, C. Biochim Biophys Acta., 2014, 1839(11), 1323-9.
	Solution Scattering and FRET Studies on Nucleosomes Reveal DNA Unwrapping Effects of H3 and H4 Tail Removal. Andresen, K., Jimenez-Useche, I., Howell, S.C., Yuan, C., Qiu, X. PLoS One., 2013, 8(11), e78587.
	DNA Methylation Regulated Nucleosome Dynamics. Jimenez-Useche, I., Ke, J., Tian, Y., Shim, D., Howell, S.C., Qiu, X., Yuan, C. Sci Rep., 2013, 3, 2121.
	Clipping of Flexible Tails of Histones H3 and H4 Affects the Structure and Dynamics of the Nucleosome Nurse, N.P., Jimenez-Useche, I., Smith, I.T., Yuan, C. Biophys J., 2013, 104(5), 1081-8.
	The Effect of DNA CpG Methylation on the Dynamic Conformation of a Nucleosome Jimenez-Useche, I., Yuan, C. Biophysical Journal, 2012, 103, 2502-2512.
	Conformational Dynamics of Titin PEVK Explored with FRET Spectroscopy Huber, T., Grama, L., Hetényi, C., Schay, G., Fül?p, L., Penke, B., Kellermayer, M.S.Z. Biophysical Journal, 2012, 103(7), 1480-1489.

光合作用

物理化學

	Photophysical Characterization of [Ir(ppy)2(dmb)][PF6] Towards Application in Light-Emitting Electrochemical Cells (LECs) Zanoni, K.P., Sanematsu, M., Murakami Iha, N.Y. Inorg Chem, 2014, 43, 162-164.
	Solid State Molecular Device Based on a Rhenium(I) Polypyridyl Complex Immobilized on TiO2 Films Patrocinio, A.O., Frin, K.P., Murakami Iha, N.Y. Inorg. Chem., 2013, 52, 5889-5896.
	A Pyrene Maleimide with a Flexible Linker for Sampling of Longer Inter-Thiol Distances by Excimer Formation Niwayama, S., Kassar, A.S., Zhao, T., Sutton, R.B., Altenberg, G.A. PLoS ONE, 2011, 6(10).

診斷學

	A Dual-Modality Optical Biopsy Approach for In Vivo Detection of Prostate Cancer in Rat Model Sharma, V., Patel, N., Shen, J., Tang, L., Alexandrakis, G., Liu, H. Journal of Innovative Optical Health Sciences, 2011, 4(3), 269-277.
	Auto-fluorescence Lifetime and Light Reflectance Spectroscopy for Breast Cancer Diagnosis: Potential Tools for Intra-Operative Margin Detection Sharma, V., Shivalingaiah, S., Peng., Y., Euhus, D., Gryczynski, Z., Liu, H. Biomed Opt Express, 2012, 3(8), 1825-1840.

ꄴ前一個：美國ISS PC1熒光光子計數(shù)光譜儀

ꄲ后一個：美國ISS 快速測試熒光壽命-磷光壽命光譜儀ChronosDFD

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